Label-free multiplexed virus detection using spectral reflectance imaging
نویسندگان
چکیده
منابع مشابه
Label-free multiplexed virus detection using spectral reflectance imaging.
We demonstrate detection of whole viruses and viral proteins with a new label-free platform based on spectral reflectance imaging. The Interferometric Reflectance Imaging Sensor (IRIS) has been shown to be capable of sensitive protein and DNA detection in a real time and high-throughput format. Vesicular stomatitis virus (VSV) was used as the target for detection as it is well-characterized for...
متن کاملHigh-throughput label-free detection of DNA hybridization and mismatch discrimination using interferometric reflectance imaging sensor.
Optical label-free biosensors have demonstrated advantages over fluorescent-based detection methods by allowing accurate quantification while also being capable of measuring dynamic bimolecular interactions. A simple, high-throughput, solid-phase, and label-free technique, interferometric reflectance imaging sensor (IRIS), can quantify the mass density of DNA with pg/mm(2) sensitivity by measur...
متن کاملHandheld imaging photonic crystal biosensor for multiplexed, label-free protein detection.
We present a handheld biosensor system for the label-free and specific multiplexed detection of several biomarkers employing a spectrometer-free imaging measurement system. A photonic crystal surface functionalized with multiple specific ligands forms the optical transducer. The photonic crystal slab is fabricated on a glass substrate by replicating a periodic grating master stamp with a period...
متن کاملMultiplexed, label-free detection of biomarkers using aptamers and Tunable Resistive Pulse Sensing (AptaTRPS).
Diagnostics that are capable of detecting multiple biomarkers are improving the accuracy and efficiency of bioassays. In previous work we have demonstrated the potential of an aptamer-based sensor (aptasensor) utilising Tunable Resistive Pulse Sensing (TRPS). Here, we have advanced the technique identifying key experimental designs for potential POC assays. The assay utilised superparamagnetic ...
متن کاملLabel-free microscopy: spectral imaging of multiphoton-excited cellular autofluorescence
A. Holloschi, HM. Kuhn, C. Müller, M. Worf, M. Rauen, T. Röder, W. Kessler, J. Mollenhauer and P. Kioschis 1 University of Applied Sciences Mannheim, Institute of Molecular and Cell Biology and Institute of Chemical Process Engineering, Mannheim, Germany 2 Steinbeis-Hochschule Berlin, Steinbeis-Insitut Multivariate Datenanalyse, Berlin, Germany 3 University of Southern Denmark, Lundbeckfonden C...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biosensors and Bioelectronics
سال: 2011
ISSN: 0956-5663
DOI: 10.1016/j.bios.2011.01.019